Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity

Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate characteristics to suit applications. In this work we report an innovative approach for immobilization a commercial lipase from Candida rugosa (CRL) which employs nanostructured carriers conjugated anti-lipase monoclonal antibodies (MoAbs). MoAbs were chemically on surface used selectively adsorb CRL molecules. Hydrolytic enzymatic assays evidenced that such technique afforded significant enhancement activity comparison free enzyme.